Evaluating Clearing Agents in Deparaffinization and Tissue Infiltration

Abstrak

The purpose of the tissue processing stage known as "clearing" is to rid the tissue of alcohol and other dehydrants. Xylene is a chemical that is frequently used as a cleaning agent in laboratories. Nonetheless, it is well recognized that xylene is extremely hazardous. This article reviews research on the role of cleaning solutions in the deparaffinization and tissue infiltration processes with the aim of identifying a safer and more effective alternative. Using secondary data from the Science and Technology Index (SINTA) database, Google Scholar, PubMed Central, and ScienceDirect, the research methodology is a literature review. As a result, thirty scholarly articles were acquired for examination. According to the findings, many synthetic and natural solvents have been shown to have cleaning effectiveness and histology quality that are on par with xylene, but at a lower risk of toxicity and tissue shrinking. However, recurrence and results might be impacted by differences in tissue response as well as procedural elements like temperature and cleaning time. Because of problems with availability and cost, some alternatives have not been extensively embraced, even though the safety profile promotes the use of ecologically friendly chemicals. Taking everything into account, integrating these findings leads to a variety of efficient cleaning solutions that strike a balance between usefulness, security, and effectiveness. This insight offers direction for upcoming laboratory research and practices that maximize histopathological tissue processing while minimizing risks to the environment and public health.