EFFECT OF DELAYING SERUM SEPARATION ON THE RESULTS OF BLOOD SUGAR FASTING EXAMINATION

Abstrak

Blood sugar fasting measurement is crucial for the diagnosis and management of diseases caused by carbohydrate metabolism disorders. One factor influencing the accuracy of blood glucose test results is the timing of serum separation. Delays in serum separation result in glycolysis, which can decrease glucose levels by 10 mg/dL per hour. The purpose of this study was to determine the effect of delayed serum separation on blood sugar fasting test results using time intervals without delayed separation, a 1-hour delay, and a 2-hour delay. The research used a simple experiment. The sample size for this study was 30 individuals, with three treatment categories no separation delay, a 1-hour delay, and a 2-hour delay, resulting in a total of 90 samples. The sampling technique used in this study was accidental sampling. Based on the results of the Kruskal-Wallis test, a p-value of 0.000 was obtained, which indicates that there is an effect of delaying serum separation on the results of blood sugar fasting examinations using a serum separation time range without delaying separation, a 1-hour delay in separation, and a 2-hour delay in separation. The average blood sugar fasting level in samples without separation delay was 91 mg/dL, after a 1-hour separation delay the average blood sugar fasting test results decreased to 76 mg/dL and at a 2-hour separation delay the blood sugar fasting test results decreased again to 65 mg/dL. Delayed serum separation greatly affects the results of blood sugar fasting tests, it is known that the longer the delay in serum separation results in a decrease in blood sugar fasting test results.